Biofilms are complex communities of microbes that are bound by an extracellular macromolecular matrix produced by the residents. Biofilms are the predominant form of microbial life in the natural environment and are the leading cause of chronic infections and difficult to treat. Our group is focused on understanding how biofilms and multicellular aggregates impact on chronic infection and we are looking for ways to treat biofilm-tolerant P. aeruginosa.

O-Specific Antigen-Dependent Surface Hydrophobicity Mediates Aggregate Assembly Type in Pseudomonas aeruginosa.
Azimi S, Thomas J, Cleland SE, Curtis JE, Goldberg JB, Diggle SP.

Allelic polymorphism shapes community function in evolving Pseudomonas aeruginosa populations.
Azimi S, Roberts AEL, Peng S, Weitz JS, McNally A, Brown SP, Diggle SP.

Competition in Biofilms between Cystic Fibrosis Isolates of Pseudomonas aeruginosa Is Shaped by R-Pyocins.
Oluyombo O, Penfold CN, Diggle SP.

Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS.
Davies SK, Fearn S, Allsopp LP, Harrison F, Ware E, Diggle SP, Filloux A, McPhail DS, Bundy JG.

Optimised chronic infection models demonstrate that siderophore 'cheating' in Pseudomonas aeruginosa is context specific.
Harrison F, McNally A, da Silva AC, Heeb S, Diggle SP.

The Pseudomonas aeruginosa PSL Polysaccharide Is a Social but Noncheatable Trait in Biofilms.
Irie Y, Roberts AEL, Kragh KN, Gordon VD, Hutchison J, Allen RJ, Melaugh G, Bjarnsholt T, West SA, Diggle SP.

The Fitness of Pseudomonas aeruginosa Quorum Sensing Signal Cheats Is Influenced by the Diffusivity of the Environment.
Mund A, Diggle SP, Harrison F.

An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms.
Harrison F, Diggle SP.

Role of Multicellular Aggregates in Biofilm Formation.
Kragh KN, Hutchison JB, Melaugh G, Rodesney C, Roberts AE, Irie Y, Jensen PØ, Diggle SP, Allen RJ, Gordon V, Bjarnsholt T.

Shaping the Growth Behaviour of Biofilms Initiated from Bacterial Aggregates.
Melaugh G, Hutchison J, Kragh KN, Irie Y, Roberts A, Bjarnsholt T, Diggle SP, Gordon VD, Allen RJ.

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection.
Roberts AE, Kragh KN, Bjarnsholt T, Diggle SP.

A 1,000-Year-Old Antimicrobial Remedy with Antistaphylococcal Activity.
Harrison F, Roberts AE, Gabrilska R, Rumbaugh KP, Lee C, Diggle SP.

Development of an ex vivo porcine lung model for studying growth, virulence, and signaling of Pseudomonas aeruginosa.
Harrison F, Muruli A, Higgins S, Diggle SP.

Quorum-sensing and cheating in bacterial biofilms.
Popat R, Crusz SA, Messina M, Williams P, West SA, Diggle SP.

Bursting the bubble on bacterial biofilms: a flow cell methodology.
Crusz SA, Popat R, Rybtke MT, Cámara M, Givskov M, Tolker-Nielsen T, Diggle SP, Williams P.

Characterisation of two quorum sensing systems in the endophytic Serratia plymuthica strain G3: differential control of motility and biofilm formation according to life-style.
Liu X, Jia J, Popat R, Ortori CA, Li J, Diggle SP, Gao K, Cámara M.

Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB.
Johansson EM, Crusz SA, Kolomiets E, Buts L, Kadam RU, Cacciarini M, Bartels KM, Diggle SP, Cámara M, Williams P, Loris R, Nativi C, Rosenau F, Jaeger KE, Darbre T, Reymond JL.

The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa.
Diggle SP, Stacey RE, Dodd C, Cámara M, Williams P, Winzer K.

Biofilm formation in Pseudomonas aeruginosa: fimbrial cup gene clusters are controlled by the transcriptional regulator MvaT.
Vallet I, Diggle SP, Stacey RE, Cámara M, Ventre I, Lory S, Lazdunski A, Williams P, Filloux A.